Flyer

International Journal of Drug Development and Research

  • ISSN: 0975-9344
  • Journal h-index: 51
  • Journal CiteScore: 46.50
  • Journal Impact Factor: 26.99
  • Average acceptance to publication time (5-7 days)
  • Average article processing time (30-45 days) Less than 5 volumes 30 days
    8 - 9 volumes 40 days
    10 and more volumes 45 days
Awards Nomination 20+ Million Readerbase
Indexed In
  • Genamics JournalSeek
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Scimago
  • Directory of Research Journal Indexing (DRJI)
  • OCLC- WorldCat
  • Publons
  • MIAR
  • University Grants Commission
  • Euro Pub
  • Google Scholar
  • J-Gate
  • SHERPA ROMEO
  • Secret Search Engine Labs
  • ResearchGate
  • International Committee of Medical Journal Editors (ICMJE)
Share This Page

Abstract

Effect of different molecular weights of chitosan on preparation and characterization of insulin loaded nanoparticles by ion gelation method

Maryam Kouchak, Mohammadreza Avadi, Mohammadreza Abbaspour, Alireza Jahangiri, Sara Kargar Boldaji

Nanoparticulate drug delivery systems have several opportunities to overcome bioavailability or stability problems of peptide and protein drugs. In this study a 23 full factorial design was used for preparation of insulin containing nanoparticles using different concentrations of low, medium and high molecular weight chitosan (CS) and tripolyphosphate (TPP) by ion gelation method. Encapsulation efficiencies (EE) of each formulation were determined by HPLC method. Regression analysis and surface plots were used in order to evaluating the effect of variables on EE and choosing the optimum formulations. The morphology of selected nanoparticles was obtained by transmission electron microscopy (TEM). Particle size, poly dispersity index (PDI) and zeta potential were also measured. Freeze-dried nanoparticles were used for drug release studies in phosphate buffer (pH=6.8). Resulted nanoparticles had mean size of 112-419 nm with PDI< 0.5 and positive zeta potential. Insulin concentration and molecular weight of chitosan had pronounced effect on EE, but chitosan concentration had no considerable effect on EE. The maximum EE of CS nanoparticles with low, medium and high molecular weights were 61.88 %, 70.89 % and 53.73 %, respectively. The in vitro drug release profiles from the low molecular weight chitosan nanoparticles showed an initial burst release followed by a slow release within 3 hours.