Shengyan Su, Jean Damascene Nsekanabo, Xinjin He, JianLin Li, Fan Yu, Meiyao Wang, Yongkai Tang
The Chinese mittens crabs (Eriocheir sinensis) has a delicate flavour and high nutritional value, making them the most important economic species in Eastern Asia (Chang, Liang, Ma, He & Sun, 2008; Chen, Zhang & Shrestha, 2007; Wu et al., 2007) With higher market demand.The present study was aimed to assess genetic diversity among the six populations of Chinese mittens crabs located in Jiangsu province.180 Chinese mittens crabs were collected in six lakes of China and in which 30 from each lakes,. The crabs were then prepared, labeled, and stored in -80°C freezer, DNA was extracted using an animal genomic DNA extraction kit (TaKaRa, Dalian).The DNA purity was tested by agarose gel electrophoresis. A total of 87 alleles were observed in 180 individuals of 6 wild Eriocheir sinensis populations. The overall mean of 6.69 alleles per locus, The loci were ranged from ( crab1 to crab21) table 2 ., Where Esin42 and HUEsa42 loci exhibited the highest number of observed alleles (15 and 13), while the lowest number of alleles was found on locus Crab2 in TH and CD populations showed a higher number of observed alleles on Esin42 and HUEsa42 loci. Fis results showed that 6 sites had negative values, and crab20 had the largest value. The results of Fit showed that the single locus showed a negative value, which was also the largest at crab20 locus. All the Fst among the populations were lower than 0.50, AMOVA analysis found that 0.36% of genetic variation came from between groups, 5.56% of genetic variation came from between individuals within a group, and 94.08% of genetic variation came from between individuals.
