Introduction: Human resources are a fundamental part of health systems and health services. Job satisfaction is a well established determinant for human health resources retention and quality of health care and health outcomes. Objectives: To investigate health professional mobility effects on health organizations effectiveness and performance, on health professionals development, training, skills acquisition and job satisfaction in a EU country (Portugal), through a survey (electronic survey) addressed to health professionals. Methods: The “Conditions of Work Effectiveness - Questionnaire-II”, validated for the Portuguese language, was selected, to evaluate the perceptions of health workers, Portuguese speakers, working in Portugal or other countries of the European Union (EU) regarding the dimensions of access to opportunity, access to information, professional support, access to resources, creativity, collaboration and activities, and global empowerment. Statistical analysis was performed using SPSS 25 (descriptive, EFA). Results: Nearly 60% of the 1800 respondents were doctors, 72.7% of them referred working in different health institutions, whereas 51.7% of nurses referred having worked in other European countries. Positive perceptions were found regarding the dimensions of “Access to Opportunity”, “Access to Information”, “Access to Support” and “Global Empowerment”. Negative perceptions were founded for the dimensions “access to resources” and “innovation /creativity (JAS)” Conclusions: Results suggested potential for intervention and implementation of policies to address the negatively perceived dimensions of access to resources and lack of appropriate rewarding, in order to improve HRH job satisfaction and contribute to quality of health care outcomes.