SYNTHESIS OF NOVEL N-SUBSTITUTED 2-(1H-BENZOTRIAZOL-1-YL) -
ACETOHYDRAZIDE DERIVATIVES AS ANTIMICROBIAL AGENTS

Jimit S. Patel; Charoo S. Garg; Dhrubo Jyoti Sen

Awards Nomination 20+ Million Readerbase

JOURNAL HOME

Editors

Articles

In Process

Google Scholar citation report

Citations : 12061

International Journal of Drug Development and Research received 12061 citations as per Google Scholar report

International Journal of Drug Development and Research peer review process verified at publons

Indexed In

Genamics JournalSeek
China National Knowledge Infrastructure (CNKI)
CiteFactor
Scimago
Directory of Research Journal Indexing (DRJI)
OCLC- WorldCat
Publons
MIAR
University Grants Commission
Euro Pub
Google Scholar
J-Gate
SHERPA ROMEO
Secret Search Engine Labs
ResearchGate
International Committee of Medical Journal Editors (ICMJE)

Share This Page

Tweets by DevelopmentDrug

- (2012) Volume 4, Issue 2

SYNTHESIS OF NOVEL N-SUBSTITUTED 2-(1H-BENZOTRIAZOL-1-YL) - ACETOHYDRAZIDE DERIVATIVES AS ANTIMICROBIAL AGENTS

Jimit S. Patel^*, Charoo S. Garg and Dhrubo Jyoti SenDepartment of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Gujarat Technological University, Arvind Baug, Mehsana-384001, Gujarat

Corresponding Author: Jimit S. PatelE. mail: jimit.india@hotmail.com

Received: 27 May 2014 Accepted: 11 June 2012

Citation: Jimit S. Patel*, Charoo S. Garg and DhruboJyoti Sen, "Synthesis of Novel N-Substituted 2-(1HBenzotriazol-1-yl)-Acetohydrazide Derivatives asAntimicrobial agents", Int. J. Drug Dev. & Res.,April-June 2012, 4(2): 322-329

Copyright: © 2012 IJDDR, Jimit S. Patel* et al.This is an open access paper distributed under thecopyright agreement with Serials Publication, whichpermits unrestricted use, distribution, andreproduction in any medium, provided the originalwork is properly cited.

Related article at Pubmed, Scholar Google

Visit for more related articles at International Journal of Drug Development and Research

Abstract

As a part of research project on the synthesis of number of substituted benzotriazole derivatives with electron donating as well as electron withdrawing groups was done and evaluated them for antibacterial and antifungal activity. First of all benzotriazole was prepared using o-phenylene diamine and sodium nitrite in acidic conditions. Now benzotriazole was reacted with ethyl chloroacetate to form benzotriazole ethyl acetate which was then reacted with hydrazine hydrate to produce benzotriazole acetohydrazide. Finally it was reacted with different sulfonyl chlorides and benzoyl chlorides to give various derivatives. The purity of all compounds have been checked by the TLC monitoring and the confirmation of the structure is checked by different spectral analysis like UV, IR, Mass and NMR and evaluated as antibacterial agent by using sulfacetamide as standard drug and for antifungal activity by using clotrimazole as standard drug.

Key words

Benzotriazole derivatives, Benzotriazole acetohydrazide, sulfonation, benzoylation, antifungal activity, antibacterial activity.

INTRODUCTION

Benzotriazole and its derivatives are important nitrogen containing heterocyclic compounds with biologically interesting properties and some pharmaceutical applications. Benzotriazole moiety has distinct property on biological system as antifungal, antibacterial, diuretics etc. Imidazole, benzimidazole and benzotriazole moiety and to screen their diverse activity like anti-inflammatory, antifungal and antimicrobial activity. For the above reasons novel series of benzotriazole derivatives had been planned to synthesize.

EXPERIMENTAL SECTION

The entire chemicals were supplied by S.D. Fine Chem. (Mumbai), Finar Chem. Ltd (Ahmedabad) and Loba Chemie Pvt. Ltd. (Mumbai). Melting points were determined by open tube capillary method and were uncorrected. Purity of compounds was checked by thin layer chromatography (TLC) on silica gel G in solvent system hexane-ethyl acetate (1:2), the spots were located under iodine vapours or UV light. IR spectra of all compounds were recorded on FT-IR 8400S Shimadzu spectrophotometer using KBr. Mass spectra were obtained using 2010EV LCMS Shimadzu instrument.

General procedure for the Synthesis of 1Hbenzotriazole:

(0.01mole) of o-phenylene diamine was dissolved in acetic acid and kept under stirring in ice-bath. Now (0.01mole) sodium nitrite was added. Temperature of reaction mixture raised to 80-85°C and automatically went down to 35°C. Then, allowed to stir for 15min. Brown coloured precipitates appeared in cold condition. The precipitates were filtered using vacuum pump and recrystallized with hot water.

Compound: 2

General procedure for the synthesis of ethyl 1H-benzotriazol-1-yl-acetate

To a solution of benzotriazole (0.01mol) in acetone, ethyl chloroacetate (0.01mol) was added. In the presence of dry K2CO3, the mixture was kept for stirring for 6 hrs. Filteration was done and the filterate was evaporated on waterbath. The resulting precipitates were recrystallized from ether. Brown needle shaped crystals were obtained.

Compound: 4

General procedure for the synthesis of 2-(1Hbenzotriazol- 1-yl)-1-(hydrazinyloxy)- ethanone

To the solution of (0.05 mole) of ethyl 1Hbenzotriazol- 1-yl-acetate in ethanol was taken in a clean and dry round bottom flask. Now 0.075 mole of hydrazine hydrate was added. After stirring for 2 hrs the reaction mixture was refluxed for 2 hrs. Now the reaction mixture was cooled in ice bath. White fibre like crystals was obtained.2

Compound: 6

General procedure for the synthesis of 2-(1Hbenzotriazol- 1-yl)-N'-[(4-substituted phenyl)- sulfonyl]-acetohydrazide

Ethyl 1H-benzotriazol-1-yl-acetate (0.001 mole) was dissolve in 10ml of pyridine in cold condition (00C) with stirring. The substituted sulfonyl chlorides (0.001 mole) were added. After addition, the reaction mixture was stirred at room temperature for 5 hrs at the end of the reaction period; the reaction mixture was poured in crushed ice. Obtained precipitates were filtered and crude was washed with cold water and cold dilute HCl. Crude was recrystallized from Ethanol.3

Compound: 7a, 7b, 7c

General procedure for the synthesis of N'- (1H-benzotriazol-1-yl-acetyl)-4-substituted benzohydrazide

Ethyl 1H-benzotriazol-1-yl-acetate (0.001 mole) was taken in a clean and dry beaker. Now 0.2 ml of substituted benzoyl chloride was added. The mixture was triturated for about 10-15 mins with the help of glass rod. Now crushed ice was added and trituration was continued. Solid product was obtained which was washed with cold water. After filtration recrystallization was done in Ethanol.

Compound: 7d, 7e, 7f

Following compounds were synthesized

Biological Evaluation

Biological evaluation holds a great significance in screening the new chemical entities. The newly synthesized compounds were screened for antifungal and antimicrobial activity.

ANTIMICROBIAL ACTIVITY

In our current study, evaluation of antimicrobial activity was carried out by using the methods mentioned below. Here responses of microorganisms to the synthesized compounds were measured with that of the standard reference drug. The standard reference drug used was sulfacetamide.

Antibacterial Activity

The microbiological assay was based upon a comparison of inhibition of growth of microorganisms by measured concentrations of test compounds with that produced by known concentration of a standard antibiotic. Two methods generally employed were turbidometric (tubedilution) method and cylinder plate (cup-plate) method. In the turbidometric method inhibition of growth of microbial culture in a uniform dilution of antibiotic in a fluid medium was measured. It was compared with the synthesized compounds. Here the presence or absence of growth was measured. The cylinder plate method depends upon diffusion of antibiotic from a vertical cylinder through a solidified agar layer in a petri dish or plate to an extent such that growth of added micro-organisms was prevented entirely in a zone around the cylinder containing solution of the antibiotics. The cup-plate method was simple and measurement of inhibition of microorganisms was also easy. Here this method was used for antibacterial screening of the test compounds.

Name of Microorganism

• Gram +Ve microorganisms

Staphylococcus aureus (MTCC No. 96)

Bacillus subtilis (MTCC No. 121).

• Gram -Ve microorganisms

Escherichia coli (MTCC No. 521).

Preparation of medium

• Nutrient agar 2%

• Peptone 1%

• Beef extract 1%

• Sodium chloride 0.5%

• Distilled water up to 100ml

All the ingredients were weighed and added to water. This solution was heated on water bath for about one and half-hour till it becomes clear. This nutrient media was sterilized by autoclave.

Apparatus

All the apparatus like petri dishes, pipettes, glass rods, test-tubes were properly wrapped with papers and sterilized in hot air oven.

Agar plate disc diffusion method

• The antibacterial activity was assayed by agar plate disc diffusion method at the concentration of 50 μg per disc.

• All the synthesized compounds were tested in vitro for their antibacterial activity against microorganisms such as Staphylococcus aureus, Bacillus subtilis (gram positive), Escherichia coli (gram negative) strains.

• Each test compounds were dissolved in dimethylsulphoxide (DMSO) to get a concentration of 10 mg/mL.

• The disc (6 mm in diameter) was impregnated with 5 μL of each test solution to get 50 μg/disc, air dried and placed on the agar medium, previously seeded with 0.2 mL of broth culture of each organism for 18 hours.

• The plates were incubated at 37 °C for 24 hours and the inhibition zones measured in mm.

• Discs impregnated with DMSO were used as a control and Sulfacetamide discs as antibacterial reference standard.

ANTIFUNGAL ACTIVITY5

Principle

The antifungal activity of all newly synthesized benzotriazole derivatives were examined against Candida albicans.

Antifungal screening of all the derivatives was done by using filter disc method.

Clotrimazole was used as a standard drug. Activity of the compounds was recorded by measuring the zone of inhibition in mm, and compared with the standard zone of inhibition produced by clotrimazole. This determination indicates whether the organism was sensitive or resistant to the compound.

Materials used

· Test organisms: Candida albicans was used for the determination of the activity.

· Growth Media: The activity was conducted on the Sabouraud dextrose agar media.

Composition

Enzymatic digest of Casein 5g

Enzymatic digest of Animal Tissue 5g

Dextrose 20g

Final pH 5.6 ±0.2 at 25 °C

Purified water 1000ml

Apparatus

Petri plate: Glass plate, which was previously sterilized by Dry Heat Sterilization was used.

Pipette: Micropipette was used for adding the required concentration of the analogues to the plates.

Glass wares: 500ml conical flask and test tubes were used.

Compounds screened: all the synthesized benzotriazole derivatives.

Solvent used: Dimethyl sulfoxide

Standard used: Clotrimazole

Preparation of standard solution

The standard drug clotrimazole was dissolved in appropriate quantity of ethanol to obtain the concentration range of 500, 750 and 1000μg/ml and the zone of inhibition was checked.

Preparation of test solution: Specified quantity (100mg) of the compound was accurately weighed and dissolved in 100ml of DMSO to get the 1000μg/ml stock solution. Further dilution was made to obtain the concentration in the range 500μg/ml, 750μg/ml and 1000μg/ml.

Procedure

· 30g of the medium was suspended in 1000ml of purified water. The mixture was allowed to boil till it forms a homogeneous solution. The medium was autoclaved at 121°C for 15 minutes at 15psi.

· Media was cooled to the temperature of approximately 40°C temperature and microorganisms were inoculated to the media. 150ml was transferred to a petri plates aseptically. Two such plates were prepared for each organism.

· Plates were allowed to cool for 20 minutes.

· Compounds were dissolved in DMSO and diluted in same to get concentration of 500μg/ml, 750μg/ml and 1000μg/ml.

· Both high and low strength discs were applied for each compound to be tested.

· The organism is reported as being sensitive if clear zone appears around both discs.

RESULT AND DISCUSSION

All the synthesized compounds were screened for their anti-bacterial activities against S.aureus, B.subtilis and E.coli and for anti-fungal activities against Candida albicans. Compounds 7a, 7b and 7e showed anti-fungal activity but less potent as compared to standard reference drug clotrimazole. Compounds 7b, 7c and 7e showed good antibacterial activity but less potent as compared to standard reference drug sulfacetamide. Compounds 7c, 7d and 7f were found to be very less potent towards anti-fungal activity.

Rest all the synthesized compounds were found to be very less potent towards anti-bacterial activity as compared to standard reference drug sulfacetamide.

Conflict of Interest

NIL

Source of Support

NONE

ACKNOWLEDGEMENT

The authors are thankful to the Quality Assurance Department of Shri Sarvajanik Pharmacy College, Mehsana for UV and FTIR spectras, Oxygen Heathcare, Ahmedabad for Mass spectras and Punjab University, Chandigarh for NMR spectras.

Tables at a glance


Table 1	Table 2	Table 3	Table 4	Table 5

Figures at a glance


Figure 1	Figure 2

5170

References

Mrunmayee P. Toraskar, Vilasrao J. Kadam, Synthesis and Antifungal activity of Azitidinone derivatives. International Journal of ChemTech Research. 2009, 1, 1194-1999
A. P. Rajput and and S. S. Rajput, Synthesis of benzaldehyde substituted phenyl carbonyl hydrazones and their formylation using Vilsmeier- Haack reaction. International Journal of PharmTech Research. 2009, 1, 1605-1611.
Deniz s. do?gruer et al., Synthesis and antimicrobial activity of some 3(2H)-Pyridazinone and 1(2H)- Phthalazinone derivatives. Turk J Chem. 2008, 32, 469-479.
Somnath Ghosh and Jhantu Das, Benzoylation of Amines sans Alkali: A Green Protocol in Neat Phase. Hindawi Publishing Corporation Organic Chemistry International. 2010,1, 1-3.
Pelczar M J., Chan ECS., Krieg NR. Microbiology; 5th edition; McGraw-Hill Book Company, New York, 1986, 687-688, 73-98.
Furniss B S. Vogel’s textbook of practical organic chemistry; 5thedition; An imprint of Addison Wesley Longman, inc., 1998, 1166-1168.
Chakraborty P A, Text Book of Microbiology; 2nd edition; New Central book agency (P) Ltd. 2005, 9- 24, 57-64.
Kokare C R, Pharmaceutical Experiments and Techniques; 2nd edition; Career Publications, 2007, 153-56.