Department of Enzymology, University of Delhi, India
Mini Review
Probing Enzyme Mechanisms: Advances in Molecular Enzymology Techniques
Author(s): Vikas Singh*
Understanding the intricate mechanisms by which enzymes catalyze biological reactions is a fundamental pursuit in the field of molecular enzymology. Recent advances in molecular enzymology techniques have revolutionized our ability to probe and decipher the complex mechanisms underlying enzyme function. This abstract highlights key advancements in the field and explores the diverse range of techniques that have significantly contributed to our understanding of enzyme mechanisms. One prominent area of progress is the application of structural biology techniques, such as X-ray crystallography, cryo-electron microscopy, and nuclear magnetic resonance spectroscopy. These methods have enabled researchers to obtain high-resolution structures of enzymes and their complexes, providing valuable insights into the active site architecture, substrate binding, and conformational changes during cat.. View More»