Elaheh Madadi Hiyagh, Sahand Mazloum Ravasan,Farshad Darvishi, Ahad Mokhtarzadeh, Behzad Baradaran
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran. University of Maragheh, Iran
Posters & Accepted Abstracts: Arch Cancer Res
Background: There are lots of treatments for cancer but always enzymes are the most efficient. Glutamine can be used to support proliferation in multiple ways. It is a proteinogenic amino acid, and can be act as a nitrogen donor for the synthesis of amino acids as well as nucleotides biosynthesis in cellular processes. The presence of L-glutaminase has been reported in various organisms, including animals, plants, and microorganisms except humans. It’s a treatment enzyme for ALL and L-glutaminase also has proved ineffectual for treatment solid tumours such as breast cancer and colorectal. In following research Yarrowia yeast glutaminase is used.
Methods: In this study Raji, MCF7 and A549 cell lines were cultured in RPMI 1640 with 10% FBS and 5% of CO2 condition. The cytotoxic effects of L-Glutaminase on Raji, MCF7 and A549 cells were studied using MTT assay. Then, flow cytometry assay was exploited to measure cell death and apoptosis stage.
Results: MTT assay showed that L-Glutaminase significantly inhibited the cell growth. According to the flow cytometry assay result, the L-Glutaminase was able to induce apoptosis in Raji, MCF7 and A549 cell lines. The apoptosis of Raji cells was more than other cell lines and A549 was more than MCF-7.
Conclusion: According to our finding, L-Glutaminase obtained Yarrowia as safe yeast could be successfully induce apoptosis in Raji, MCF7 and A549 cell lines. Therefore, it could be used as a novel and safe therapeutic candidate for cancer treatment.
E-mail:
perzhad.mazloumi@gmail.com
Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi 