Anti-viral effects of ethyl acetate fraction from Distylium racemosum against Newcastle disease virus

Newcastle disease virus (NDV) is a member of the Paramyxoviridae family, a negative-sense RNA virus. NDV causes not only serious infectious disease in birds but also zoonosis. In addition, despite vaccination, NDV outbreaks are growing in the worldwide. Therefore, NDV is a serious public health concern worldwide. The aim of this study was to evaluate the anti-viral effects of the fractions from Distylium racemosum (D. racemosum) that naturally grows on Jeju Island, against the NDV, a Newcastle disease (ND)-inducing virus. The cell viability of fractions from D. racemosum was determined by the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide (MTT) assay. To study its anti-viral effects, hemagglutination (HA) titer and cytopathogenic effect (CPE) reduction assay were conducted using a lentogenic NDV strain. Expression levels of viral gene were determined by quantitative reverse transcription–polymerase chain reaction (qRTPCR). Among various fractions (hexane, ethyl acetate, butanol and water fraction), ethyl acetate fraction showed the anti-viral effects against NDV. The results showed a concentration-dependent inhibitory effect of ethyl acetate fraction treatment on HA titer, CPE and expression levels of viral gene. Based on these results, ethyl acetate fraction from D. racemosum can be used as an effective candidate material for the development of treatment for NDV.

Biography :

Hye-Ran Kim has graduated from Catholic University of Pusan as Doctor of Science. Later on she obtained her Post-graduation from Catholic University of Pusan and then started studying at Catholic University of Pusan where she has continued her research. Presently, Presently she is studying at Busan City

E-mail: hrkim308@gmail.com

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