Flyer

Archives of Clinical Microbiology

  • ISSN: 1989-8436
  • Journal h-index: 24
  • Journal CiteScore: 8.01
  • Journal Impact Factor: 7.55
  • Average acceptance to publication time (5-7 days)
  • Average article processing time (30-45 days) Less than 5 volumes 30 days
    8 - 9 volumes 40 days
    10 and more volumes 45 days
Awards Nomination 20+ Million Readerbase
Indexed In
  • Open J Gate
  • Genamics JournalSeek
  • The Global Impact Factor (GIF)
  • Open Archive Initiative
  • China National Knowledge Infrastructure (CNKI)
  • Directory of Research Journal Indexing (DRJI)
  • OCLC- WorldCat
  • Proquest Summons
  • Publons
  • MIAR
  • University Grants Commission
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
  • Google Scholar
  • Scimago Journal Ranking
  • Secret Search Engine Labs
  • ResearchGate
  • International Committee of Medical Journal Editors (ICMJE)
Share This Page

Isolation and antibiotic susceptibility pattern of methicillin resistant bacteria species associated with items carriage from supermarkets in Akure, Ondo State, Nigeria

Euroscicon conference on Bacteriology and Mycology
June 18 - 19 , 2018 Paris , France

Festus A Olajubu, Christianah D Oluyede, Timothy O Adejumo and itilayo Ogunyemi

Adekunle Ajasin University, Akungba-Akoko, Nigeria

Posters & Accepted Abstracts: Arch Clin Microbiol

Abstract:

Colonisation by methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis (MRSE) of objects being commonly shared among people has generated a lot of public health concerns. With the spread of supermarkets and hypermarkets across the world, people are brought together and thus facilitate transmission of microbes either directly through hand to hand contact or indirectly through inanimate objects such as shopping trolleys and shopping baskets within the supermarkets. This study focused on investigating the presence of MRSA and MRSE on carriage items from Shoprite (SHP), Ceci (CC), OK (OUK) and Feelbest (FB) supermarkets in Akure, Nigeria and the genes responsible for their resistance were determined through conventional polymerase chain reaction (cPCR). Swabs samples were obtained from handles of trolleys and baskets used by customers. A total of 253 samples were collected and each sample was immediately transferred into 5ml of peptone water. The samples were processed using standard microbiological method. Bacterial growths were identified based on cultural, morphological and biochemical characteristics. Susceptibility test of the isolates was done on Mueller-Hinton agar. MRSA and MRSE were detected using Cefoxitin (1μg) and were further tested by cPCR to determine the resistant genes using five different primers. The study shows that out of the 353 isolates identified, 37.1% were S.aureus, 49.6% were S.epidermidis, 5.7% were Klebsiella spp, and 7.6% were E.coli. Ten (10) MRSA and Five (5) MRSE isolates possess MecA, Sul1, Aph, blaM, AmpC and EreA genes. Out of the sites sampled, SHP carriage items had the highest microbial contamination (70.5%) whereas FB carriage items had the lowest. The prevalence of 2.8% MRSA reported from this study requires measures to curb the possible adverse health implications. Regular disinfection of trolleys and educating people to improve hand washing habits are recommended as measures to decreasing transmission of MRSA, MRSE and their infections.