Sahand Mazloum Ravasan, Elaheh Madadi Hiyagh, Farshad Darvishi, Ahad Mokhtarzadeh and Behzad Baradaran
Tabriz University of Medical Sciences, Iran University of Maragheh, Iran
Posters & Accepted Abstracts: Arch Cancer Res
Background: Cancer is one of the most important problems in the world. Today Enzymes have been intensively studied as a source of antitumor compounds. L-Asparaginase (L-ASNase) is one of the most therapeutic enzyme which used for the cancer therapy. The attendance of L-ASNase has been reported in various organisms, containing animals, plants, and microorganisms (bacteria, fungi, algae, yeast and actinomycetes) except in humans. L-ASNase enzyme which isolated from Yarrowia yeast was used for this study
Methods: In this study, Raji, MCF7 and A549 cell lines were cultured in RPMI 1640 with 10% FBS and 5% CO2 condition. Cytotoxic effects of yeasts L-asparaginase was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay.Then, flow cytometry assay was exploited to measure cell death and apoptosis stage.
Results: According to our findings, yeasts L-asparaginase can inhibit cell growth in a time and dose dependent manner. Flow cytometry assay result showed that yeasts L-asparaginase was able to induce apoptosis in Raji, MCF7 and A549 cell lines. The apoptosis of raji cells is more than other cell lines and A549 is more than MCF-7.
Conclusion: Our results showed that yeasts L-asparaginase could successfully induce apoptosis in Raji, MCF7 and A549 cell lines. Therefore, it could be used as a novel and safe therapeutic candidate for cancer treatment.
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