As an alternative to array-based, gel-based, stable isotope tag or label-based methodologies, label-free mass spectrometric protein quantification methods have been developed to overcome the difficulties associated with differential expression proteomics. In this article, we concentrate on the problems with label-free techniques that use quantification based on peptide ion peak area measurements. Chromatographic alignment, peptide qualifying for quantification, and normalisation are some of these problems. In order to solve these problems, we present a number of methods that are combined in a recently created label-free quantitative mass spectrometry platform. These methods allow for thorough, precise, and repeatable protein quantitation in highly complex protein mixtures from experiments with numerous sample groups. We give a number of scenarios where the platform was successfully used to evaluate diverse protein expression or abundance in body fluids, in vitro Nano toxicity models, tissue proteomics in genetic knock-in mice, and cell membrane proteomics as illustrations of the usefulness of this approach.
KeywordsBiomedical Research; Mass spectrometry; Two-dimensional electrophoretic; Quantification
Published Date: 2023-04-28; Received Date: 2023-04-01
