Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Mini Review
Biomedical Research Using Mass Spectrometry
Author(s): Abolgasam Jouban*
As an alternative to array-based, gel-based, stable isotope tag or label-based methodologies, label-free mass spectrometric protein quantification methods have been developed to overcome the difficulties associated with differential expression proteomics. In this article, we concentrate on the problems with label-free techniques that use quantification based on peptide ion peak area measurements. Chromatographic alignment, peptide qualifying for quantification, and normalisation are some of these problems. In order to solve these problems, we present a number of methods that are combined in a recently created label-free quantitative mass spectrometry platform. These methods allow for thorough, precise, and repeatable protein quantitation in highly complex protein mixtures from experiments with numerous sample groups. We give a number of scenarios where the platform was successfully us.. View More»
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