Senior Research Scientist, Jai Research Foundation, Research & Development Section, JRF Global, Valvada, Gujarat-396105, India
Research Article
The trans-differentiation of Keratinocyte: Requisite for skin wound healing and cosmetic surgery
Author(s): Dr. Mamata Mishra*
Objective: Skin wound healing involves the interaction of epidermal and dermal
cells, growth factors, and cytokines. Keratinocyte is the most dominant cell type
in the epidermis and plays multiple roles for skin repair function. Cultured human
primary keratinocytes are required for the treatment of burns, cutaneous wounds
diabetic foot ulcers, and cosmetic therapy
Methods: Human primary keratinocytes from different age groups were isolated,
characterized and efficacy was analyzed. Primary human keratinocytes were
purchased from commercially available sources and their trans-differentiation
properties were studied.
Results: Serum-free media with high calcium was used to isolate keratinocytes
from different donors with varied age groups. The number of keratinocytes isolated
from the total popul.. View More»
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