Department of Science, Zootechnics and Ispezione degli Alimenti, sez. B. Ferrara, University di Napoli Federico II, Via Delpino 1, 80055-Napoli, Italy
Editorial
A Note on the Classification and Confirmation of reference genes
Author(s): Graciela Jannice*
Normalization, which is typically accomplished by comparing the abundance of the gene of interest to that of an endogenous reference gene, is an essential step in gene expression analysis for obtaining useful data from reverse transcription quantitative PCR (RT qPCR) assays. It's not easy to find these reference genes, which should be stable when expressed in multiple tissue samples and under different experimental conditions. A set of genes has been identified and evaluated in this work that could serve as a reference for gene expression studies on water buffalo. The first step involves downloading a Bos taurusexpressed sequence tags database from the TIGR Gene Index and mining it with simple frequency algorithms to determine which tentative consensuses are more suitable for inclusion in a starter set of candidate reference genes because they are present in a remarkable number of.. View More»
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