R.S. Mehta Jain Department of Biochemistry and Cell Biology, KBIRVO Block, Vision Research Foundation, Sankara Nethralaya, Chennai, India
Research Article
Simultaneous Determination of Homocysteine, Cysteine, Glycine, Glutamic Acid, Methionine, Serine and Taurine in Human Plasma by Using Reverse Phase-High Performance Liquid Chromatography
Author(s): Muthuvel Bharathselvi*, Karunakaran Coral and Konerirajapuram N Sulochana
Alterations in the plasma aminothiol levels can be considered as biomarkers for the diagnosis and screening of human disorders namely cardiovascular diseases. Among the common method, ELISA is available for homocysteine estimation, the analytical method like HPLC is considering as a gold standard. In the proposed study, we have developed a RP-HPLC method for the simultaneous determination of homocysteine, cysteine, glycine, glutamic acid, methionine, serine and taurine in human plasma using fluorescence detector. Separation was achieved on C18 column with gradient elution using 80% methanol, 0.05 M acetate buffer (pH-7.0) and the column was maintained at 26°C and the mobile phase was pumped at flow rate of 1.0 mL min-1. The detection of eluent was carried out at fluorimetrically (excitation 340 nm, emission 450 nm) with a total run time 30 min. The calibration curve ranged from 25.. View More»
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