Shenzhen Academy of Peptide Targeting Technology at Pingshan and Shenzhen Tyercan Bio-Pharm Co, China
Research Article
The Suppressive Effects of Cytotoxic Agents on Lymphoma Cell Proliferation and Tumor Growth
Author(s): Natalya I. Vasilevich*, Lichun Sun*, Mengli Yang, Haihua Xiao, Jinping Yang, Kunxian Feng, Chengfang Jian, Tuan Ma, Andre H. Sun and Alexander V. Chestkov*
Objective: Ansamitocin P3 (AP3) and paclitaxel as tubulin inhibitors and
camptothecin (CPT) as a Topo Ⅰ inhibitor are considered to be among the most
active natural products. We investigate the roles and mechanisms of AP3, paclitaxel
and CPT in inducing apoptosis in human histiocytic lymphoma U937 cells.
Methods and materials: In vitro studies included CCK8 assay to estimate
antiproliferative activity and flow cytometry to analyze apoptosis of U937 cells.
qRT-PCR was revealed the change in gene expression leading to apoptosis. In vivo
experiments were performed with xenograft tumor model using five weeks-old
female SCID mice.
Results: CCK8 assay exhibits AP3 as a great antiproliferative activity compound with
a half-maximal inhibitory concentration (IC50) at 0.18 ± 0.04 nM, while .. View More»
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