Department of Virology, University of Ibadan, Ibadan, Nigeria
Review Article
Loop-Mediated Isothermal Amplification: A Rapid Tool for Microbial Diagnosis
Author(s): Sheriff Agbaje*
The contributions of nucleic acid amplification to the field of medical science cannot be overemphasized. Polymerase Chain Reaction (PCR) has been the most preferred technique of nucleic acid amplification over the years as a result of its high sensitivity. However, PCR limitations which include high cost of set up and high level of expertise needed makes it a setback to many researchers. Loop Mediated Isothermal Amplification (LAMP) was introduced to fill these bridges. In this review, previous studies of LAMP are highlighted, laying more emphasis on its types, usage, detection of its amplicons as well as its merits and demerits. LAMP advantages include its simplicity, cost effectiveness, rapidity, specificity and direct use of sample from site of infection. The various application of LAMP is also discussed. We have concluded that Since LAMP conform to the criteria set by the World H.. View More»
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