Department of Chemistry and Biochemistry, University of Jinan, China
Review
Modern Developments and Emerging Directions in Hydrophilic Interaction Chromatography-Mass Spectrometry for Metabolomics and Proteomics
Author(s): Shivanshi Singh*
Proteomics and metabolomics have drawn more interest during the past ten years than any other -omics methods. Both methods have matured to the point where they are relevant in many clinical applications, including as the discovery of biomarkers, enhanced illness diagnosis, staging, and prognosis, as well as a greater understanding of many physiological processes. Due to its simplicity and reproducibility, reversed-phase liquid chromatography mass spectrometry is regarded analytically as the gold standard in proteomics and metabolomics. The complexity of the proteome cannot be resolved by RPLC-MS alone, because highly polar metabolites are often poorly preserved. Due to its orthogonal separation process, hydrophilic interaction chromatography in this context constitutes an appealing supplementary technique. This review provides a summary of the research literature regarding the use of .. View More»
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